![]() More information about setting up reactions. Set up ddPCR reaction mix with primers or probes, sample, Bio-Rad supermix ( probes or EvaGreen). Run times need to be coordinated with CQLS Core staff during lab open hours. The system can be operated as a Multi-user instrument, or Full service with sample dropoff for CQLS to run. ![]() The Bio-Rad QX200™ AutoDG™ Droplet Digital™ PCR System is located in ALS 3012. After PCR the samples are transferred to a QX 200 Droplet Reader where individual droplet intensities are read. Hydrolysis probes (Taqman) or EvaGreen (SYBR-like) chemistry can be used on the system. ![]() The system includes an Automated Droplet Generator (ADG) that generates 20,000 droplets from one ddPCR reaction containing your target or background DNA. Samples are processed 8 at a time. Pathogen Detection and Microbiome Analysis Researchers are utilizing ddPCR to quantify genomic alterations, such as copy number variation, detect rare sequences, and determine absolute quantification of target DNA copies without the need for a standard curve. Droplet Digital PCR (ddPCR) affords scientists the ability to quantify template molecules that may be undetectable using traditional techniques. The Bio-Rad QX200™ AutoDG™ Droplet Digital™ PCR System is a technology that provides high-precision with absolute quantification of nucleic acid targets. Information Pertinent to the Bio-Rad System Below If you are starting a new digital PCR project contact Anne-Marie Girard for more information about setting up your project for the new system. We are obtaining a Thermo Fisher QuantStudio Absolute Q dPCR System in mid-July for multi-users and will be retiring our Bio-Rad QX200 Droplet Digital system as a service in the near future. Biological Data Science (BLDS) Graduate minor.Advanced Cyberinfrastructure Teaching Facility.Phylogenetic Inference & Population Genetics.
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